Size Exclusion Chromatography Free Essays

Size Exclusion Chromatography Size Exclusion Chromatography (SEC) is the separation technique based on the molecular size of the components. Size exclusion chromatography is a kind of method to separate different size of molecules that put in solution. It was first discovered by two scientists who named Grant Henry Lathe and Colin R Ruthven. We will write a custom essay sample on Size Exclusion Chromatography or any similar topic only for you Order Now Both of them received the John Scott Award for this fabulous invention. There are various applications for Size exclusion chromatography such as biochemical aspect and polymer synthesis. For application in biochemical aspect, this technique can find out the quaternary structure of purified proteins which possess slow exchange times, since it can be carried out under native solution conditions and preserve macromolecular interactions. The reason why we use this technique for purification is Size exclusion chromatography is a low resolution chromatography method as it does not identify similar species very well. It can also test the tertiary structure of protein as it measures the hydrodynamic volume, allowing folded and unfolded versions of the same protein to be distinguished. Besides using in biochemical research, it is able to find the distribution of the sizes of polymer molecules like if a solvent is chose and run, we can create a calibration curve to determine the sizes of polymer molecules in it. It is better to introduce the mobile phase and stationary phase first. Stationary phase is the solid absorbent or the pore(SEC) with solid support that allow sample across through it while the mobile phase is the sample percolate through or along to the stationary phase. In SEC, separation is achieved by the differential exclusion from the pores of the packing material, of the sample molecules(mobile phase) as they pass through a bed of porous particles(stationary phase). For the principle of the SEC, molecules of different sizes can be separated by this technique because of differential time spent inside a solid phase particle which excludes entrance of relatively larger molecules, allows some entrance of medium-sized molecules, and allows free accessibility of the smallest molecules. The particles contain pores with tunnels(stationary phase) in which the size can be controlled depending on the size of molecules(mobile phase) to be separated. Smaller molecules experience a more complex pathway to exit the particle than do larger molecules. Because molecules that have a large size compared to the pore size of the stationary phase have very little entrance into the pores, these larger sized molecules elute first from the column. Medium sized molecules are relatively large compared to the pore size of the solid phase and therefore may find some pores in which they enter and spend some time. Smaller-sized molecules have more pores that are accessible to them and therefore spend more time inside the pores relative to larger-sized molecules. Therefore, smaller molecules elute last and larger molecules elute first in SEC. â€Å"Elute† is mean that the carrier of the mobile phase or the mobile phase from chromatographic bed emerge. For the pore size, which is the important part of stationary phase in SEC, solid phase materials used in SEC are usually classified based on their ability to separate different sizes of proteins. Since size is a difficult item to accurately measure for a large molecule, the solid phase materials are identified with a molecular weight range instead and the weight is equated with size. All compounds with a molecular weight less than or equal to the lower number in the range will see the entire internal volume of the beads resulting in no selection and therefore no separation. All compounds with a molecular weight greater than or equal to the higher number in the range are completely excluded from the inside of a bead and therefore no separation is achieved. Molecules with weights or sizes between these two extremes of the range can be separated. This is the numerical pore size range reported for each solid phase material used in SEC. The pore size used for a separation is dependent on the size range of the particular set of molecules to be separated. Smaller pore sizes are used for rapid desalting of proteins or for protein purification. Intermediate pore sizes are used to separate relatively small proteins. Very large pore sizes are used for purification of biological complexes. For the factor that affect the SEC, first, the particles in solution do not have a fixed size, resulting in the probability that a particle that would otherwise be hampered by a pore passing right by it. Second, the stationary-phase particles are not ideally defined, both particles and pores may vary in size. . The stationary phase may also interact in undesirable ways with a particle and influence retention times, though great care is taken by column manufacturers to use stationary phases that are inert and minimize this issue. Third, increasing the column length will enhance the resolution, and increasing the column diameter increases the capacity of the column. Proper column packing is important to maximize resolution: An over-packed column can collapse the pores in the beads, resulting in a loss of resolution. An under-packed column can reduce the relative surface area of the stationary phase accessible to smaller species, resulting in those species spending less time trapped in pores. Unlike affinity chromatography techniques, a solvent head at the top of the column can drastically diminish resolution as the sample diffuses prior to loading, broadening the downstream elution. The advantages of this method include good separation of large molecules from the small molecules with a minimal volume of eluate, and that various solutions can be applied without interfering with the filtration process, all while preserving the biological activity of the particles to be separated. Second, the technique is generally combined with others that further separate molecules by other characteristics, such as acidity, basicity, charge, and affinity for certain compounds. Third, with size exclusion chromatography, there are short and well-defined separation times and narrow bands, which lead to good sensitivity. The SEC is separated rapidly. Then, there is also no sample loss because solutes do not interact with the stationary phase. The stationary phase doesn’t have any absorbent that nteract with the sample and carry out the reaction with the sample. For the disadvantage of the this method , first is the molecular mass that we need to know. The SEC separation is base on the molecular size/ weight, like the gel electrophoresis. It is required to know that there are the range for different of the molecular size. If the difference of the molecular size in the mobile phase, it is not recommended to use this separation. So, before using the SEC, the molecular size o f each sample in mobile phase are required to identify. In addition, the accommodated of SEC is limited. The mobile phase can not be too big. The time scale of the chromatogram is short, and, in general, there has to be a 10% difference in molecular mass to have a good resolution Also, the pore size need to be determined, too small size or too big size will lead to the failure of the separation SEC. In the world, the chromatography is the separation of the sample base on the polar, size, acidity, basicity, charge, and affinity for certain compounds†¦ Size Exclusion Chromatography is the one of the chromatography that base on the size of the sample, which is similar to the principle of gel electrophoresis. One different point is the stationary phase, which is the column with the pores of the particles. Reference: (http://www. separations. us. tosohbioscience. com/ServiceSupport/TechSupport/ResourceCenter/PrinciplesofChromatography/SizeExclusion/) (http://www. asdlib. org/separations_pdfs/Size_Exclusion_Chromatography_Separations_Module-finalversion. pdf) (http://en. wikipedia. org/wiki/Size-exclusion_chromatography),goldbook How to cite Size Exclusion Chromatography, Essay examples

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Table of Contents Source Problem Secondary problems Issues Regarding Human Resource Management Succession of Cyrus Mistry Acquisition of Corus Analysis of the Secondary Problems Issues Regarding Human Resource Management Succession of Cyrus Mistry Acquisition of Corus Criteria of Evaluation Alternatives Decentralization of Management Appointment of Sole Chairman Mentorship by Ratan Tata Support from the Senior Directors and Management Horizontal growth Backward Integration Recommendation and Justifications Implementation and Monitoring References Source Problem Tata Group is a global corporation which was founded by Jamsetji Tata in1868. Headquarter of Tata Group is in India and it is operating in more than hundred countries across 6 continents. The main seven sectors of business activities undertaken by the Tata Group contains services, materials, energy, engineering, consumer products, chemicals, communication and information system. Tata group has 100companies operating worldwide and the total of Tata companies was 103.27 billion USD in 2013-14. Various companies of Tata Group have already achieved global leadership through excellent operations. According to a UK based consultancy firm, Brand Finance Tata ranks 34 among the top 500 most valuable brands across the world. Tata group has adopted several expansion strategies under the leadership of Ratan Tata. In steel, automotive, hotel industry and mining sector, Tata has implemented its expansion strategy through acquisition. In this paper, the issues encountered by Tata Group in regard to the global expansion strategy will be discussed. Both hardship and success has been experienced by Tata group in several phases of the expansion. Some projects are undertaken in the rural areas and the situation is not viable for the company. Hence, the major challenge of the Tata Group is to empower the group companies for surviving in the difficult global economic scenario without compromising with the vision, mission and values of the Tata Group. Secondary problems Tata Group has more than hundred companies and each business is an independent working entity. The group has been encountering several short terms as well as long terms issues in pursuit of the global expansion. In this section, major short term as well as long term problems have been indentified and discussed in brief. Issues Regarding Human Resource Management Presently, Tata group has an employee base of 571,470 people across the world. Human resource is the key driver for the success of a company. The global expansion strategy of Tata Group involves merger and acquisition. When two entities merge, the cultural difference of the employees leads to various issues. As the company is aiming to achieve organic as well as inorganic growth trough future expansions, human resource management and retention of the talents will be a major challenge for the company. Succession of Cyrus Mistry After retirement of Ratan Tata, Cyrus Mistry has taken the charge of the company. The successor will confront challenge for revving up some of the flagship companies of the Tata group. the stakeholders are anxious to evaluate the performance of the new Chairman of Tata Group. The major question is whether Cyrus Mistry will be able to continue the success story of the group. Another question is whether he would be able to take Tata to higher position or maintain the current status. Acquisition of Corus In 2007, Tata Steel ad acquired one of the Steel giant Corus group as a part of the global expansion strategy. But, Corus Group has significant burden of debt have been major problem for Tata Steel. The performance of Tata Steel Europe is not up to the mark. Hence, acquisition of Corus Group has leaded to a major problem for the Tata Group. Analysis of the Secondary Problems The major problems of encountered by Tata Steel have been identified in the above section. This section will focus on analyzing the major problems so that the issues of global expansion strategy can be addressed properly for finding solutions. Issues Regarding Human Resource Management In the dynamic global business environment Tata Group has been facing challenges regarding effective management of the human resource. As the operations of the group have been expanded in the foreign territories through merger and acquisition management of diverse workforce has become a significant challenge for the Tata group. The strong philosophy of the Tata Group has been embedded within the operations and activities of the company. The company tends to adopt a model which was practiced by the predecessor of rattan Tata. This model suggests that the flagship companies of the Tata Group will have their own chairman. Presently, Cyrus Misrty has been given the sole authority to choose the governance model. The major challenge of the group is to ensure that the employees of the merged entities and the newly appointed workforce follow the culture and values of the Tata group. Succession of Cyrus Mistry In 2011 Cyrus Mistry was appointed as the Depuy Chairman of The Tata Group prior taking over the charge of the group as Chairman in 2012. He had been putting great effort to understand the business for continuing the elevation process. Cyrus Mystry is responsible for formulating strategies in order to achieve the organizational goals regarding profitability. Presently, the company needs to focus on the global market position of the group as well as its profitability. He is also responsible for ensuring that the traditional values as well as ethical standards of the company are maintained. However, Cyrus Mystry is encountering various challenges regarding the market position of some flagship companies. It has been observed that performance of Tata Motors is not up to the mark and it is losing its market share. In terms of passenger vehicles, Mahindra Mahindra has captured the market by introducing new SUV models and technologies. Sales of Tata Safari and Sumo have been affected. Additionally, sales of Tata Nano have been far from the forecast. Volkswagen and Ford have been found to be the biggest rival of Indigo which was utilized for boosting up the automobile sales of the Tata Group. Among all the flagship companies of Tata Group, Tata Teleservices (TTSL) has been contributing highest in the loss of the group. In 2012-13 the net loss of the Tata Teleservices was reported to be 48580 million (in rupees). In 2013-14, Tata Teleservices has reported the highest loss of 61660 million INR. Tata Power is also encountering issues due to the high coal price and depreciation of INR. It is encountering loss for the past few years. However I the first quarter of the financial year 2014-15, the net loss is narrowed down According to various stakeholders, Cyrus Mistry will encounter challenges due to lack of experience. Cyrus Mistry has limited exposure of the present business structure, strategy and operations of the Tata Group as he has only five years experience as a non executive director. In order to avoid the conflict of interest, Cyrus had already disassociated himself from the family business. Hence, Cyrus Mistry will encounter various issues regarding decision making, rejuvenating the sick flagship companies, mentoring and formulating strategies. Acquisition of Corus Management of Tata Steel indicates acquisition of Corus as a big milestone for the company. The major aim of this acquisition was to penetrate the mature market of Europe. The technological benefits can be achieved from this acquisition. Even after 7 years after acquisition of Corus by Tata Steel, the financials of Tata Steel Europe does not indicate a good position. Though Europe has been improving its economic condition after the long recession, the financial performance of Tata Steel Europe has not been found to be satisfactory. Turnover of Tata Steel Europe for the financial year 2013-14 is reported to be 3% lower compared to the previous year. The reason is decrease in the average revenue per tonne leaded by the deterioration in the market condition in the first of the FY 2013-14. It has been observed that the Earnings before Income Tax Depreciation and Amortization (EBTIDA) has increased year in year basis, however fallen sequentially. The net loss of the year has decreased from the quarter of previous year but increased in comparison to the previous quarter. According to the report of Kotak Securities, though production and delivery of Tata Steel Europe has increased, there is a sharp decline in the profit margins and tagged as grossly underperforming. The average realization of Tata Steel Eurpoe was decreased by 7.7% sequentially and the cost of raw materials increased by 0.1% simultaneously. This situation leaded to a sharp fall of 25.5% in EBTIDA per tonne. RAMACHANDRAN, K. 2011. 'Cyrus Mistry Needs To Address A Number Of Explicit, Implicit Challenges'. Tata.com,. 2008. 'New Challenges, New Opportunities Via @Tatacompanies'. Criteria of Evaluation Tata is a value driven organization and the growth has been directed by the core values of business which include integrity, understanding, excellence, unity and responsibility. The goals and objectives of the group are the major factors for determining the strategic orientation and plans. In order to evaluate the performance of the company, the financial performance of Tata Group has indicated that the total revenue of the company has increased by 18.5%. Increase in total asset is 20.6%. The global operation has been improved and international revenues have increased by 27%. Hence, for the next few years, Tata Group needs to set a minimum target of 18.5% for evaluating the revenue growth of the entire group. Additionally, the company must set a target for Tata Steel to become top three companies of the world. As the Tata group emphasizes on the values and social responsibilities, it must continue the with the social responsibility projects across the globe for enhancing its goodwill. Alternatives In the above sections, the major problems of Tata Group have been indentified and analyzed along with empirical evidences. This section has recognized some alternatives for addressing the existing issues. Decentralization of Management Decentralization of management structure will be suitable for Tata Group and support the global expansion strategy. Human resource management is a major challenge for the group of companies. Engagement of the local employees will be beneficial for the Tata Group. Individual Chairman can be appointed for the flagship companies of Tata Group. Appointment of Sole Chairman Cyrus Mistry will be the sole Chairman of the Tata Group. He will be responsible for handling the issues of Tata group. As individual chairman will be appointed for the flagship companies, the burden will be reduced for Cyrus Mistry. Mentorship by Ratan Tata Ratan Tata can help Cyrus Mistry in the strategic decision making and act as mentor. It will help in the smooth succession of Cyrus Mistry and he will also get easily accepted by the stakeholders. Support from the Senior Directors and Management Cyrus Mistry needs support from the senior director who has been working for longer period and holding senior positions in the management of Tata Group. Their support will helps to understand the strategic direction of the company along with its values and philosophy. Horizontal growth It has been observed that the Tata Steel Europe has not been able to achieve the desired performance. Sustainable growth can be achieved through competitive advantages. Significant resources gained from Corus can be utilized for product innovation which will contribute in the horizontal growth of Tata Steel. Superior RD of Corus can successfully accomplish the horizontal growth which will result in broadening the profitability. Backward Integration Backward integration suggests an organization to internally produce various parts of its supply chain. In case of Tata Steel, backward integration refers to own iron ore mines to supply in its business. Tata Steel can consider acquiring mines for obtaining the desired quality raw material at a lower price. Thus Tata Steel will have lower operating cost which will provide competitive advantage to the company and improve EBTIDA of Tata Steel. However, this strategy is capital intensive. Recommendation and Justifications Finally, the following recommendations are provided along with justification for confronting the challenges: Horizontal growth can be achieved through innovation. Strong RD department of Corus can assist in this process for meeting the customer demands. Thus the Tata Group will be able to improve its performance in the dynamic global business environment. Tata Steel can consider acquisition of iron ore mines which will reduce the cost of production. Hence, backward integration can aid in the improvement of EBTIDA. Implementation and Monitoring The implementation of these strategies needs an effective implementation plan. The Chairman of Tata Steel is recommended to develop an implementation plan along with the senior management team. As backward integration is a capital intensive strategy, it must be evaluated and implemented effectively so that the desired outcome is achieved. Appointing individual chairman for each flagship company will face obstacle from the stakeholders. Hence, plan needs to be designed for encountering obstacles during the implementation of the recommended strategies. All these activities need to be monitored by analyzing the financial as well as operational performance of the Tata Group as well as individual flagship companies under Tata Group. If any gap is identified in this process, it must be coordinated and change the plan accordingly. References Baggonkar, Swaraj. 2013. 'Challenging Road Ahead For Tata Motors: Cyrus Mistry'. Businesstoday.intoday.in,. 2014. 'Tata Power's Net Loss Narrows To Rs 111.30 Crore On Fall In Forex Losses - Chatterjee, Dev. 2014. 'Tata Teleservices Posts Record Rs 6,166-Cr Loss'.Business Standard. Dutt, Ishita Ayan. 2014. 'Tata Steel's Problem Child Struggles To Make Good'.Business-Standard.Com. Economictimes.indiatimes.com,. 2014. 'Tata Steel Directors Report | Hill, Charles W. L, and Gareth R Jones. 2014.Strategic Management. 1st ed. Mason, OH: South-Western, Cengage Learning. Philip, Lijee, and Satish John. 2013. 'Ratan Tata's Lieutenants To Be Chairmen At Tata Steel, Tata Motors, TCS And Indian Hotels - RAMACHANDRAN, K. 2011. 'Cyrus Mistry Needs To Address A Number Of Explicit, Implicit Challenges'. Tata.co.in,. 2014. 'Leadership With Trust Via @Tatacompanies'. Wheelen, Thomas L, J. David Hunger, and Thomas L Wheelen. 2008.Concepts In Strategic Management And Business Policy. 1st ed. Upper Saddle River, NJ: Pearson Prentice Hall. Wheelen, Thomas L, J. David Hunger, and Thomas L Wheelen. 2008.Concepts In Strategic Management And Business Policy. 1st ed. Upper Saddle River, NJ: Pearson Prentice Hall. Hill, Charles W. L, and Gareth R Jones. 2014.Strategic Management. 1st ed. Mason, OH: South-Western, Cengage Learning.

Difference Between Self Esteem and Self Confidence free essay sample

* Difference between self esteem and self confidence Self confidence and self confidence are linked but they are not the same thing. Your self esteem is how much you like yourself, how you see yourself. What we see when we look in the mirror. Self esteem is somewhat passive and isnt something others can observer in you. While, if we look at, self confidence. It is something more active, and describes our abilities and willingness to interact with the world around us. By comparison, we can see how self confidently somebody (or even a group such as a ports team) is acting. Self esteem is the idea about yourself. It is how much you value yourself and how important you think you are. It is the acceptance, respect, trust and belief in you. Self confidence means that you believe in your own abilities. We will write a custom essay sample on Difference Between Self Esteem and Self Confidence or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page Self confidence is related to self assurance, you are proud of your own abilities. You are positive, take risks and set goals and reachable standards. With self-confidence you have the certainty that you can do it . Self esteem is valuing oneself unconditionally, if you are alive you can have full self- esteem. Go on, you have permission. Self-esteem is valuing yourself as a human being. You are alive, you have the powers of thought, emotion, speech movement. You can do things! When you think about the amazing freedom of being alive and being able to think, feel, speak and behave as you want, this is awe inspiring. No matter what you do or who you are you can esteem yourself. Self-confidence is having the confidence that you can do this or that thing. It is about your skills and performance and is conditional. If you are competent at a certain skill, you can be confident that you can do that. Confidence is conditional upon you being good at the said skill. If you are not good at it, there is no use being confident. If you are confident that you can skydive buy youVe never done it before and know nothing about it, that is recipe for disaster. Your confidence will improve naturally as your competence increases. There is another element that you can be confident in that can help you improve your confidence at any skill.

The Unavoidable Evil free essay sample

Procrastination is an unavoidable evil in my life. Last night I was attached to You Tube for two hours. I didn’t do my any homework and failed to finish my essay. Was this a wise option? NO. I should have shut off my computer after only a half hour but, I was already hooked. I had completed my math homework by the time I switched on the glowing screen to my laptop that night, but nothing else of value had been accomplished. In these two hours of â€Å"homework† I learned about the â€Å"dangers† of women in overalls, and about the obsessive qualities of a teenage boy. The evils of You Tube had entranced my once again, every so often it works it’s magic, if its not gay men talking about absolutely nothing of importance, it’s a 12 year old stalker, or an overly obsessed Brittany Spears fanatic. Why do I watch these brain numbing episodes on the highly popular webpage? I’m bored, I’m sick of the homework I have been assigned night after night and I want to accomplish absolutely nothing in life†¦well maybe not the last one. We will write a custom essay sample on The Unavoidable Evil or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page This is a prime example of senioritis. Too bad, that procrastination has plagued me since the first day of my high school career. You may then ask yourself, â€Å"Then, what is the key to her success?† The answer, late nights and short essays.

City of Thieves Essay Sample

City of Thieves Essay SampleCity of Thieves is an online adult game in which players assume the role of detectives trying to solve crimes and apprehend the criminals. The online version of the game has received much attention because of its unique storyline and the multi-player mode. If you are planning to download a City of Thieves essay sample to help teach you about the game, make sure that you are using a clean and legal copy of the game.The game is basically based on a novel written by James Patterson. You are free to read the novel at your own pace. You can play it whenever you want to without any restrictions. The story of the game was actually inspired by the Patterson novel City of Fire.Your first copy of the City of Thieves essay sample may not be too effective. The makers of the game have tried to make the copy as user-friendly as possible. They try to add many features and elements that will help you learn the basics of the game and become a more skilled player. Most peop le find it quite challenging to come up with sentences that contain the right number of words.There are many tips that are usually found in the tutorials that are easy to understand and will help you be able to use them easily. You should know that the tutorial will not always work well for everyone. That is why you should learn the basic rules of the City of Thieves before you proceed to the next step.There are various articles that will also help you understand the basics of the game. For this, you need to go to the City of Thieves website. You will find a tutorial and different articles that will guide you in using the online game. After you have read all the tips and articles, you can then start practicing and playing the game. After learning all the basic rules, you will be able to become a good player and also be able to use the right words when you play.It is important to note that City of Thieves essay sample is for educational purposes only. If you plan to take the game ser iously, you will need to buy the games at online stores. These shops will give you a copy of the game for a low price. You can then enjoy the game and learn a lot while you are playing it.One thing that you should remember is that if you want to gain a lot of skills when you are playing City of Thieves essay sample, you will need to spend a lot of time practicing. The game has many aspects that require the players to be very attentive. If you are not keen enough to devote enough time to learning, you may find it difficult to perform well when you play the game. You will need to practice several times to be able to achieve perfect scores and win the games.Another important thing that you should remember is that City of Thieves essay sample is not an easy game. If you are looking for a fun and exciting experience, then you should choose a different game such as Frontline: European War.

Dylan Thomas Essays - Dylan Thomas, Ferris, , Term Papers

Dylan Thomas Dylan Thomas' Final Trip to America Dylan Marlais Thomas was born in Swansea, Wales on October 27, 1914. He died November 9th, 1953 in New York City. In only 39 years, Dylan Thomas left an indelible mark on history. Thomas published numerous books of his poetry, plays, short stories, and various other works. He first toured America in early 1950, reading at a variety of public forums. This tour was very successful and Thomas fell in love with America, a romance that would bring his end just more than three years later. "This first lecture tour of three months was a roaring success, or roaring and a success" (Sinclair, 166). Thomas gave great lectures on this tour, but more importantly, he discovered New York City. "New York seemed to egg him on" (Ferris, 232). Dylan grew fond of "some of the seedier Irish bars of Third Avenue and particularly...the White Horse Tavern" (Sinclair, 164). Dylan was even forced to leave his hotel because of "drunkenness" (Ferris, 232). Thomas had gained a reputation of being a heavy drinker and he wasn't ready to disappoint his American followers. "Dylan lived up to his roistering and shocking reputation, while turning in some of his greatest performances as a lecturer" (Sinclair, 166). "He was loudly applauded," Ferris wrote, "His rich voice overcame any problems of meaning. People frequently said that Thomas' way of reading made them understand poems for the first time; but it may be under the influence of his voice, the literal meaning of a poem became irrelevant" (233). After only two readings in New York, Dylan moved on to tour the rest of the country. Thomas moved west stopping in major cities all over America to lecture by day and drink by night. Tales of his growing dependence on alcohol preceded him. The quality of Dylan's lectures deteriorated as he moved west on his tour of American bars. For the final five weeks of his visit to America, Thomas returned to New York (Ferris, 248). Dylan described his last two weeks in New York as a "liquid, libidinous fortnight" (Ferris, 250). Thomas admitted to having three affairs during the trips (Ferris, 250). He left for home on June 1st, 1950. When Thomas arrived at his home in Laugharne, Wales he discovered that his trip to America did not prove to be lucrative enough to support his poetry for any extended period of time (Ferris, 252). Being at home, with his wife, Dylan recuperated from the wear and tear of his first tour (Sinclair, 173). "He wrote that he had to ruin his health again because he felt so preposterously well. Such were the healing powers of Laugharne - and of love" (Sinclair, 175). During this period at home, Dylan's father became ill and "Dylan was shocked into his last fertile period of writing poetry" (Sinclair, 175). By the end of 1951, Thomas had agreed to take another lecture tour of America, This time however, his wife Caitlin, having received news of his love affairs on his last tour, insisted on going (Sinclair, 176). This tour went on with moderate drinking by Thomas. Caitlin assisted by spending whatever money Dylan earned. The second and the first trips were very similar in form. Dylan left For Wales on May 15, 1952. Thomas returned to Laugharne and his Father's condition worsened. DJ Thomas died on December 16; Dylan's own health was beginning to deteriorate. Years of heavy drinking, heavy smoking, and hard living were beginning to take their toll just two months after his 38th birthday (Ferris, 287). Thomas was suffering from many ailments, several pulmonary in nature (Ferris, 287). Despite all of this, needing money, Thomas agreed to tour America for a third time (Ferris, 288). He left on April 21, 1953. Thomas soon began an affair with a woman he was working with named Liz Reitell (Ferris, 289). Dylan was receiving opportunities in America, but was "finding it difficult to produce anything at all" (Ferris, 291). Reitell and Thomas fell in love and Liz soon became worried about his drinking problem. He fell down a staircase while drunk and broke his arm. Reitell took him to see Dr. Milton Feltenstein who set his broken bone and treated him for illnesses held over from England (Ferris, 292). Thomas returned to England, as scheduled, a few days later. Dylan's condition quickly deteriorated. He decided to return to America shortly after coming home. Many feel that this was an attempt at self-destruction. His body could not take another trip to America, but Thomas

Writing a Book for Your Business

WRITING A BOOK FOR YOUR BUSINESS Thinking of writing a book? Writing expert Wintress Odom, owner of The Writers for Hire, says you need to answer a few essential questions before you get started. In her interview with the host of The Price of Business, Wintress discusses how to determine whether writing a book would be beneficial to your business. Specifically, she talks about: †¢ The different reasons for writing a book. †¢ Setting realistic expectations. †¢ Focusing your thoughts. †¢ Other mediums that can support your book. Click the play button  below to listen to the interview. document.createElement('video'); https://www.thewritersforhire.com/wp-content/uploads/2016/08/POB_Interview_Wintress-Book.mp4